Fig. 4: H1 clamp vaccination induces cross-protection in ferrets.
From: Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
A Vaccination schedule and dosing outline for the study. B Weight loss, C virus neutralisation from serum collected on day 49, virus titres detected in D nose swabs, E lung tissue and F nasal turbinates and G histology evaluation of lung tissue from ferrets challenged intranasally with the H1N1pdm09 virus (A/Netherlands/602/2009). H Weight loss, I survival, J virus titres in throat swabs, K viral load in nasal turbinates, L viral load in lung tissue and M histology evaluation of lung tissue from ferrets challenged intratracheally with the H5N1 virus (A/Indonesia/5/2005). Data is representative of geometric mean with error bars representing the SD. For virus neutralisation and ELISA graphs: ***p < 0.0005, ****p < 0.0001, determined using one-way ANOVA with Tukey’s multiple comparison post hoc test. For throat and nose swab viral titre graphs: *p < 0.05, **p < 0.005 compared to PBS-immunised animals on the corresponding sampling day, as measured by repeated measures two-way ANOVA with Dunnet’s test for multiple comparisons.
