Fig. 3: Antibody responses presented as EC50 values determined by ELISA.

Sheep immunised with either empty BPs indicated by white, B-cell epitope peptide indicated by light grey, B/T-cell epitope peptide indicated by grey, B-cell epitope-coated BPs indicated by dark grey and B/T-cell epitope-coated BPs indicated by black. Statistical significance was determined by Nonparametric Mann–Whitney (Minitab 19) when p < 0.05. * indicates statistical significance compared to empty BPs and epitope peptides. ** indicates statistical significance between B- and B/T-cell epitope-coated BPs. Eight individual sheep serum samples per group were analysed as each three technical replicates. a shows antibody titre representing binding to empty BPs quantified by ELISA. b shows antibody titre representing binding to B-cell epitope-coated BPs quantified by ELISA. c shows antibody titre representing binding to B/T-cell epitope-coated BPs quantified by ELISA. All data are mean and error bars represent +/–SD.