Fig. 2: Antibody response to MVA SARS-CoV-2 vectors in mice. | npj Vaccines

Fig. 2: Antibody response to MVA SARS-CoV-2 vectors in mice.

From: MVA vector expression of SARS-CoV-2 spike protein and protection of adult Syrian hamsters against SARS-CoV-2 challenge

Fig. 2

Mice were immunized subcutaneously with 105, 106, or 107 PFU of MVA-S, MVA-ssM, MVA-RBD, or MVA (five5 per group) and boosted with the same vector dose at 3 weeks following the first immunization. a ELISA analysis of sera from MVA-S immunized mice using a full-length S capture antigen. b ELISA analysis of sera from mice immunized with all three MVA-S-expressing vectors at the 107 PFU vector dose using RBD as a capture antigen. c ELISA analysis of sera from mice immunized with all three MVA-S-expressing vectors at the 107 PFU vector dose using S as a capture antigen. d Sera from vector-immunized mice tested for antibody isotype by ELISA. Standard deviations are shown by error bars. Numbers above some bars (e.g., 4/5) indicate the number of seropositive mice of the total number of animals. e Neutralizing antibody analysis of sera from mice immunized twice with a 107 PFU dose tested using a lentiviral vector pseudotype neutralization assay. *P < 0.1, **p < 0.01, and ****p < 0.0001.

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