Fig. 4: Efficacy of VSV-KFDV vaccines against lethal KFDV challenge. | npj Vaccines

Fig. 4: Efficacy of VSV-KFDV vaccines against lethal KFDV challenge.

From: A live-attenuated viral vector vaccine protects mice against lethal challenge with Kyasanur Forest disease virus

Fig. 4

Groups of 12 BALB/c mice were injected intraperitoneally with 104 PFU VSV-KFDV at day-56 and -28 (prime-boost group) and at day-28 (prime only). The animals were challenged with 1000LD50 (10 PFU) of KFDV at day 0 and monitored over a period of 42 days. Control animals were immunized with 104 PFU of VSV-EBOV vaccine at the same time points and challenged the same way. a Weight curve VSV-KFDV1. b Survival curve VSV-KFDV1. c Weight curve VSV-KFDV2. d Survival curve VSV-KFDV2. e KFDV load in brain tissue. Four mice in each group were euthanized at 8 dpi and KFDV brain titers were determined by a TCID50 assay. Error bars represent the standard deviation. Statistical significance for survival curves and brain titers were analyzed using the Mantel–Cox and the unpaired T tests in Prism 7 (GraphPad), respectively. Significantly different results are indicated as ***p < 0.001, and ****p < 0.0001.

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