Fig. 6: Passive transfer of hyperimmune plasma protects mice against lethal KFDV challenge.
Female BALB/c mice (n = 10 per group) were treated intraperitoneally (IP) with 500 µl of pooled plasma from mice that were immunized prime/boost with either VSV-EBOV or VSV-KFDV1. Twenty-four hours after treatment, all mice were challenged IP with 100 LD50 (1 PFU) of KFDV. On 8 dpi, 4 mice from each group were euthanized for sample collection. Surviving mice were monitored until 42 dpi. The KFDV-prM-E IgG titer of the pooled plasma used for treatment was determined to be 3 log10. a Weight curve. b Survival curve. c KFDV load in brain tissue. Error bars represent the standard deviation. Statistical significance for survival curves and brain titers were analyzed using the Mantel–Cox test and the unpaired T tests in prism 7 (GraphPad), respectively. Results are indicated as *p < 0.05 and ****p < 0.0001.
