Table 1 ZIKV RNA loads in maternal and fetal tissues in marmosets^a.

ID	Treatment	Challenge^b	Fetus^c	F. Hd^d	F. Bd^e	Placenta	M. Spl^f	M. mLN^g
C1	None	0	2/2	4–67	2–7.5	284	15.5	2120
V1	ZPIV 2x	20	1/4	1.2	BLQ^h	+/− ⁱ	0.0^j	0.0^j
V2	ZPIV 2x	26	1/3	BLQ^h	BLQ^h	BLQ^h	0.0^j	0.0^j
V3	ZPIV 2x	36	0/2	0.0^j	0.0^j	0.0^j	0.0^j	0.0^j
V4	ZPIV 2x	72	0/3	0.0^j	0.0^j	0.0^j	0.0^j	0.0^j

^aZIKV RNA load is presented as indicated number multiplied by 10⁵ copy per gram tissue.
^bAll marmosets were i.m. challenged with ZIKV-BR at indicated weeks after prime dose vaccination.
^cNumber of ZIKV RNA-positive fetuses per total number of fetuses.
^dF. Hd, fetal head.
^eF. Bd, fetal body.
^fM. Spl, maternal spleen.
^gM. mLN, maternal mesenteric lymph nodes.
^hSamples contained detectable signals for viral RNA; however, the level was below the limit of quantitation (BLQ, Ct value > 35) as described in Materials and Methods.
ⁱPlacental tissues were sampled at three different locations, RNA was isolated from each piece separately, tested for RT-PCR in duplicate of each origin of tissue. One site was positive (2.52 × 10⁷ copy g⁻¹ tissue) for ZIKV RNA whereas the other two sites were negative. No infectious virus particles were detectable after co-culture of placental homogenates with Vero cells and U937 cells (Supplementary Fig. S1).
^jViral RNA was not detectable and considered below the limit of detection (Ct value > 37). The tissue tpe-specific limit of detection (log10 copy number g⁻¹) are 3.8 in the fetal head, 4.2 in the fetal body, 3.4 in the placenta, 4.11 in the spleen, and 3.08 in the LN.

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