Fig. 9: RNAseq analysis reveals IN BReC-CoV-2 vaccination results in unique gene expression signatures enriched for T cell responses. | npj Vaccines

Fig. 9: RNAseq analysis reveals IN BReC-CoV-2 vaccination results in unique gene expression signatures enriched for T cell responses.

From: Intranasal administration of BReC-CoV-2 COVID-19 vaccine protects K18-hACE2 mice against lethal SARS-CoV-2 challenge

Fig. 9

All analyses were performed on CLC genomics workbench 21. a Number of significant (FDR p < 0.05) activated and repressed genes in IN BReC-CoV-2 and IM BReC-CoV-2 groups. b Volcano plot indicating significant gene expression profile of IN BReC-CoV-2 compared to NVC. Red circles denote upregulated genes and blue circles represent downregulated genes. c Heat maps were generated by Morpheus. Heat map represents gene counts of immunoglobulin genes in each mouse lung. d Significant fold changes of the immunoglobulin genes of interests in both IN and IM BReC-CoV-2 groups. e Heat maps were generated by Morpheus. Heat map represents gene counts of adaptive immune response genes of interest in the mouse lung. f Significant fold changes of adaptive immune response genes in both IN and IM groups. IM BReC-CoV-2 did not have significant fold changes. Red asterisks next to the sample ID indicate mouse morbidity before the termination of the study. NVC3 and IN1 euthanized on day 6, IM5 euthanized on day 7, IM3 euthanized on day 8, IM2 euthanized on day 9, and NVC6 and 7 euthanized on day 10. g Gene set enrichment analysis of IN BReC-CoV-2 compared to NVC was performed on WEB-based Gene SeT AnaLysis Toolkit. The enrichment ratio of significant GO-terms compared to the number of genes in each enriched gene set. Red represents activated genes and blue represents repressed genes.

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