Fig. 1: Design of plasmid vaccines.
From: Two DNA vaccines protect against severe disease and pathology due to SARS-CoV-2 in Syrian hamsters
a Diagram showing the pIDV-II vector and antigens. Both plasmids encode codon-optimized full-length SARS-CoV-2 spike (S) open reading frame in the presence or absence of 5mer4 at 3′ end. b Detection of SARS-CoV-2 Spike protein in HEK 293 cells lysate by western blot. Cells were transfected with pIDV-II-SARSoCoV-Spike_v1 (pIDV-V1) and pIDV-II-SARSoCoV-Spike_v5 (pIDV-V5). The mock sample represents the 293 T cells transfected with empty vector pIDV-II; Western blot was performed using a convalescent human serum.
