Fig. 2: Humoral and cell-mediated responses in vaccinated mice.
From: Two DNA vaccines protect against severe disease and pathology due to SARS-CoV-2 in Syrian hamsters
a BALB/c and b C57BL/6 mice were immunized with pIDV-II-SARS-CoV2-Spike-V1 (pIDV-V1), pIDV-II-SARS-CoV2-Spike-V5 (pIDV-V5), or sham vaccination with buffer only (control). Spike-specific antibodies was detected by ELISA. All mice received two doses of vaccine, one on day 0 and another on day 28 (n = 4 per group per timepoint). Each dose of vaccine was administered via electroporation (EP) following intramuscular injection of 50 µg/dose (ug DNA by IM + EP route). (sera dilution 1:400). **** indicates P value = <0.0001 as determined by one-way ANOVA. Error bars represent standard deviation. T cell response was analyzed by ELISpot 10 days after boost in c BALB/c and d C57BL/6 mice. Sham immunized mice were used as control. Splenocytes cell suspension were stimulated with SARS-CoV2 peptide pools encompassing the entire SARS-CoV2 Spike glycoprotein. No significant difference was observed for the cell-mediated response between vaccinated animals with two versions of vaccines.
