Fig. 1: Generation and characterization of first-generation MV-based candidate ZIKV vaccines. | npj Vaccines

Fig. 1: Generation and characterization of first-generation MV-based candidate ZIKV vaccines.

From: Measles-based Zika vaccine induces long-term immunity and requires NS1 antibodies to protect the female reproductive tract

Fig. 1

a MV-ZIKV vaccine constructs and controls. b Immunofluorescence staining of Vero cells were infected at MOI-0.1 for 72 h with the recovered MV-ZIKV candidate vaccines and control viruses. The permeabilized cells were stained for MV using an α-MV nucleoprotein mouse monoclonal and for the ZIKV-E using a mouse monoclonal antibody (Biofront 1176-56). The MV-N cl25 antibody (Sigma, NP cl.25) is conjugated with a Dylight 488 (green color changed to Yellow for visualization) fluorophore and ZIKV-E is stained with a secondary goat α-mouse Cy-3 (Jackson Immunoresearch, red color changed to Cyan for visualization) antibody. Confocal images were taken using NIKON-A1R, 60X magnification, 3X zoom. The scale bar measures 30 μm. The data is representing one replicate of n = 5. c Sucrose-purified virions were analyzed on SDS-PAGE (10%) stained with SYPRO Ruby. Zika virus PRVABC59 strain was loaded as the control. The data is representing one replicate of n = 4. d Western blot analysis of sucrose-purified virions probed for ZIKV-E (Biofront mouse mAb) and MV-H (Rabbit polyclonal sera). Zika virus PRVABC59 strain was loaded as the control. The data is representing one replicate of n = 4. e Western blot analysis of cell lysates of VERO cells infected with MV-ZIKV candidate vaccine at MOI-5 for 60 h. Zika virus PRVABC59 infected cell lysates were loaded as the control. The blot was probed for ZIKV-E (Biofront mouse mAb) and MV-N (Sigma, NP-Cl25). The data is representing one replicate of n = 2.

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