Fig. 1: ConSOSL.UFO expression form SIV-based transfer vectors. | npj Vaccines

Fig. 1: ConSOSL.UFO expression form SIV-based transfer vectors.

From: Persistent immunogenicity of integrase defective lentiviral vectors delivering membrane-tethered native-like HIV-1 envelope trimers

Fig. 1

a 293T Lenti-X cells were transfected with SIV-based transfer vectors pGAE-UFO.664 and pGAE-UFO.750 or with pConSgp160, expressing parental ConSgp160 Env. Cells were membrane (top panels) or intracellular (bottom panels) stained with bNAb 2G12 and analyzed by CSLM. b 293T Lenti-X cells transfected with pGAE-UFO.750 were membrane stained with trimer specific PGT145 (panels 1, 2) or PGDM1400 (panels 3, 4) bNAbs and analyzed by CSLM. Anti-human IgG Alexa Fluor 488 was used as secondary Ab. Nuclei were stained in blue by DAPI. Scale bars, 10 μm. Images represent single central optical sections. Shown are results from one representative of n = 3 experiments.

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