Fig. 4: IDLV-UFO.750 is pseudotyped with native-like trimeric envelope. | npj Vaccines

Fig. 4: IDLV-UFO.750 is pseudotyped with native-like trimeric envelope.

From: Persistent immunogenicity of integrase defective lentiviral vectors delivering membrane-tethered native-like HIV-1 envelope trimers

Fig. 4

a 293T Lenti-X cells producing IDLV-UFO.750 were probed with 2G12 (top panel) or PGT145 (bottom panel) bNAbs and observed by TEM. Four representative images are shown for each bNAb staining. Bars, 0.2 μm. b CLSM observation of GFP-labeled IDLV-UFO.750 obtained by adding a plasmid expressing SIVGag protein fused to GFP during production. Purified particles were immuno-stained with anti-Env 2G12 bNAb (top panel) or trimer-specific PGT145 bNAb (bottom panel) and anti-human IgG Alexa Fluor 647 secondary Ab (red). Top panels show the merged images in which the yellow dots represent the overlapping green (SIVGag-GFP) and red (UFO.750) signals. Bottom panels represent the individual fluorescent images. Two representative images for each Ab staining are shown. Bars, 5 μm. Shown are results from one representative of n = 2 experiments.

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