Fig. 3: Effects of PyTRAP monoclonal antibodies on parasite activity.
a Each mAb was assessed for in vitro function of inhibition of invasion and traversal. In each case, mean values of % inhibition (i.e., 100% – invasion or traversal value) from the 100-µg/mL mAb concentrations (bar plots with these and additional conditions shown in Suppl. Fig. 1) are represented on a color axis. b Binding kinetics for each mAb was measured by BLI and shown as kinetic maps with gray dashed diagonal contour lines labeled with the corresponding Kd values and symbols representing the characterized epitopes for invasion (left) and traversal (right) inhibition. Higher-affinity (i.e., those possessing lower Kd values) mAbs are closer to the upper-right corner of this plot. Symbol color coding represents “strong” inhibition for mean values ≤50%, “mild” inhibition for values ≤70%, and “weak” for mean values >70% observed at the 100-µg/mL concentration. The red box highlights the region of the kinetic plots containing the values for mAbs that showed strong inhibition in invasion and traversal assays. c Summarized sterile protection ratios following passive-transfer-challenge experiments (number of animals in each group is shown below the corresponding bar, individual values shown in Table 1). For c, * is p ≤ 0.05 and **** is p ≤ 0.0001; values reported were not adjusted for multiple comparisons due to small group sizes and limited comparisons.
