Fig. 5: MVA-VLP-SUDV elicits antibodies capable of inducing multiple Fc-dependent innate immune effector functions. | npj Vaccines

Fig. 5: MVA-VLP-SUDV elicits antibodies capable of inducing multiple Fc-dependent innate immune effector functions.

From: A single immunization with a modified vaccinia Ankara vectored vaccine producing Sudan virus-like particles protects from lethal infection

Fig. 5

Sera collected at day 27 (after the first vaccine dose) and at day 54 (after the second vaccine dose) were assessed for Fc-dependent antibody functions when stimulated with SUDV GP protein. Guinea pig sera were incubated with GP coated fluorescent beads which were then incubated with either human neutrophils a or THP-1 monocytes b. Cells were then analyzed by flow cytometry to quantify phagocytic intake of fluorescent bead/antibody complexes. GP bound beads were incubated with guinea pig sera and exposed to guinea pig complement c; and C3 deposition (MFI of FITC) was measured by flow cytometry using a fluorescent anti-guinea pig C3 antibody. Mean values ± SEM. N = 5 animals per group. P values determined by 2-way ANOVA followed by Sidak’s multiple comparison test. N = 5 animals per group. ADNP, antibody-dependent neutrophil phagocytosis; ADMP, antibody-dependent monocyte phagocytosis; ADCD, antibody-dependent complement deposition.

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