Fig. 1: Humoral & cellular immune responses induced by VOC-based subunit vaccine formulations.

SDS-PAGE (reducing conditions, 3 µg total protein, Coomassie stain, a) and UPLC-SEC profiles (b) of SmT1v3 reference strain ‘R’, Beta ‘B’ and Delta ‘D’ generated for vaccine formulations. Black and white arrows indicate trimer and hexamer protein populations, respectively, based on molecular weight estimates by MALS. C57BL/6 mice (n = 10/group) were immunized i.m. with SmT1v3 (R, B, and/or D) adjuvanted with SLA or AddaS03 on days 0 and 21. Serum was collected and analyzed by ELISA against tagged SmT1-R to determine the antibody titers on days 20 and 28 (c, d, respectively). Grouped data are presented as geometric mean + 95% confidence interval. Splenocytes were harvested on day 28 and analyzed by IFN-γ ELISpot when stimulated by spike peptide pools (e). Values obtained with media alone were subtracted from those measured in the presence of the peptides. Grouped data is presented as mean + standard error of mean (SEM). Statistical significance of differences for groups receiving formulations with the same adjuvant vs. the equivalent dose of SmT1v3-R is shown: *p < 0.05 & **p < 0.01 by one-way ANOVA followed by Tukey’s multiple comparisons test.