Fig. 4: Assessment of antibody-dependent activation of cell-mediated cytotoxicity by plasma collected post infection. | npj Vaccines

Fig. 4: Assessment of antibody-dependent activation of cell-mediated cytotoxicity by plasma collected post infection.

From: Moderate to severe SARS-CoV-2 infection primes vaccine-induced immunity more effectively than asymptomatic or mild infection

Fig. 4

a MRC-5 cells were gated on forward and side scatter characteristics to illustrate expression of Wuhan-Hu-1 S on non-transduced (white histogram, 0%) and spike-transduced (gray histogram, 92%) MRC-5 cells in a representative histogram. Background NK cell-mediated lysis of b non-transduced (white bar) or S-expressing (grey bar) MRC-5 cells mediated either by plasma Ab from SARS-CoV-2-seropositive or seronegative (pre pandemic) individuals, respectively, was measured. Bars represent the mean of individual plasma samples (circles) with standard deviation depicted in red. Natural killer cell-mediated lysis of S-expressing MRC-5 cells enabled by Ab generated after infection was measured by 51Cr release (E:T 25:1) and correlation with c DPI/DPSO and d symptom severity assessed. COVID-19 symptom severity: asymptomatic = 0; mild = 1; moderate = 2; severe = 3. ADCC experiments were performed with three independent PBMC donors and representative plots shown.

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