Fig. 3: Passive transfer of immune serum can protect from lethal RVFV.

Naïve mice (n = 3–5 per experimental group) were inoculated with 200 uL of serum from vaccinated mice, either undiluted or diluted 1:5, 1:10, 1:20, 1:100, or 1:500. Control mice were inoculated with normal mouse serum. All mice were bled then challenged with WT RVFV. A Endpoint titers of RVFV-specific antibodies were determined via ELISA (B) and FRNT (C) 1 day prior to challenge. Mice were then challenged with 2 or 200 TCID₅₀ of WT RVFV and monitored for survival (D). Terminal viral RNA load (E) and ELISA titer (F) were also measured. Open circles in E&F represent animals that died or required euthanasia while closed circles represent animals that survived; the dotted line is the LOD of the assay. For ELISA and FRNT₈₀ graphs the dotted line is the LOD (100 for ELISA, 40 for FRNT₈₀). Negative values were plotted at 50 and 20, respectively. Geometric means and geometric standard deviation are noted.