Fig. 4: Antiviral cellular immunity elicited by multiple RhAd52.CoV.Consv prime / boost regimens in BALB/c mice.

a–c Antiviral cellular immunity as measured by IFNγ ELISpot (A) and IFNγ-secreting CD8⁺ CD44⁺ T cells in isolated lung (b) and spleen (c) tissue from BALB/c mice immunized with the designated prime RhAd52 vaccine, boosted with the corresponding RhAd52 vaccine 4 weeks later, and sampled 4 weeks following the boost (8 weeks following the prime immunization). Individual mice are denoted by single dots with bars displaying the mean ± the SEM. Gating strategy and representative flow plots are found in Supplementary Figs. 1–4.