Fig. 3: AMP-CpG immunization induces lymph node transcriptional reprogramming reflecting APC recruitment with increased potential for antigen processing and presentation. | npj Vaccines

Fig. 3: AMP-CpG immunization induces lymph node transcriptional reprogramming reflecting APC recruitment with increased potential for antigen processing and presentation.

From: Amphiphile-CpG vaccination induces potent lymph node activation and COVID-19 immunity in mice and non-human primates

Fig. 3

C57Bl/6 J mice (n = 2) were immunized once with 10 µg WH-01 RBD protein and 1 nmol soluble or AMP-CpG. a Schema showing animal dosing and experimental schedule. b, c, f Heatmap representation of whole lymph node mRNA analyzed by NanoString nCounter® Mouse Immunology Panel. Shown are mock-subtracted, average Z-scores of gene transcript levels significantly (p < 0.05 as determined with Rosalind software) downregulated (≥−1.5-fold change, blue) or upregulated (≥1.5-fold change, red) at 6 h (b), 24 h (c), and 72 h (f) post-injection relative to mock immunization. Insignificant values with p ≥ 0.05 are shown in gray. Genes were clustered into 11 groups (box insert) using Gene Ontology databases. Volcano plot representation of log-transformed transcript values from soluble CpG (d) and AMP-CpG (e) immunized animals at 24 h depicted in c. Mock vaccines contained PBS vehicle only. Statistical analysis was performed using Rosalind software. Dotted horizontal line represents significance threshold of p = 0.05 (determined with Rosalind software); vertical dotted lines represent fold-change limits of ±1.5-fold change. LN lymph node.

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