Fig. 4: Optimized production of Pseudotyped VSV incorporating NiV-HeV-EBOV glycoproteins. | npj Vaccines

Fig. 4: Optimized production of Pseudotyped VSV incorporating NiV-HeV-EBOV glycoproteins.

From: Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses

Fig. 4

A Different amounts of DNA (µg) were used to give the closest range for each target protein for optimized production detected by WBA. B WBA detection of proteins in cell lysate of cells transfected to produce the pseudotyped VSV particles. C Shows the best DNA ratio for production of pseudotyped VSV detected by WBA. D The cell surface expression (CSE) of the target proteins analyzed by FC. E Detection of the target proteins on pseudotyped VSV using FV. F A micrograph of the produced pseudotyped VSV particles viewed using a FEI T20 electron microscope. G Flow cytometry data showing an example of the presence of double-positive viral populations (red) as compared to the negative control bald particles (blue). H Percentage of viral populations double positive for various pairs of glycoproteins with extracellular tags. One representative experiment of three is shown.

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