Fig. 5: Carbohydrates preserve the thermostability of pseudotyped VSV particles. | npj Vaccines

Fig. 5: Carbohydrates preserve the thermostability of pseudotyped VSV particles.

From: Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses

Fig. 5

A Vero cell entry capabilities for pseudotyped VSV incorporating monovalent and multivalent target proteins determined using Renilla Luciferase assay. The relative MOI of the pseudotyped VSV was determined based on luminescence produced from the Luc gene and read as relative light units (RLU). The pseudotyped VSV virions were collected 40 h post transfection. The monovalent and multivalent pseudotyped VSV virions were diluted 1:100 to 1:1,000,000 and their entry capabilities determined to assess the optimal entry levels for the monovalent and multivalent pseudotyped VSV. B Determination of Vero cell entry of non-lyophilized multivalent pseudotyped VSV exposed to 4 °C, 25 °C, and 37 °C for 42 days. C Determination of sucrose and trehalose capability to preserve the multivalent pseudotyped VSV during lyophilization. D Determination of viability for the 5% w/v trehalose preserved pseudotyped VSV. Viability was determined using the Renilla Luciferase assay.

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