Fig. 9: Analysis of hamster survival, weight, and temperatures during the course of challenge experiments.

A Percent survival of test-vaccinated vs. mock-vaccinated hamsters, following challenge with live NiV, HeV, or maEBOV. Hamsters vaccinated with the test vaccine survived challenge with all the three virulent viruses except that one hamster died from a lymphoma (unrelated cause). Those vaccinated with mock VSV virions were euthanized at critical control points after signs of disease. B Weight records for the test vs. mock vaccinated hamsters groups. Hamsters in the test vaccine group showed minimal variation on weight during the course of experiments while those in the mock-vaccinated groups registered significant weight loss prior to euthanasia. C Body temperature records for the mock vs. test-vaccinated groups. Hamsters test-vaccinated showed minimal variation in body temperatures while the variation was marked for the mock groups. D Clinical scores for the multivalent pseudotyped VSV vaccinated hamsters compared the mock-treated group. Clinical scores were based on a 15-point scale that includes scoring appearance, respiratory signs, mobility, temperature, and neurological signs such as paralysis, seizures, and appearing moribund. Additive scores of 15 or greater required immediate consultation with the veterinarian to determine animal disposition. There was marked deviation of clinical scores between test and mock vaccinated groups. Solid lines represent test-vaccinated animals, while dashed lines represent mock-vaccinated animals.