Fig. 3: Protection and viral load in pCTV-WS immunized K18-hACE2 mice. | npj Vaccines

Fig. 3: Protection and viral load in pCTV-WS immunized K18-hACE2 mice.

From: Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concern

Fig. 3

Body weight a and survival b of pCTVØ (black lines) or pCTV-WS (red lines) immunized mice and challenged with the Wuhan strain of SARS-CoV-2 [n = 8 mice/group]. c Lung viral load in pCTVØ (black bar) or pCTV-WS (red bar) immunized K18-hACE2 mice and challenged with the Wuhan strain at 5 DPI [n = 4 mice/group]. d SARS-CoV-2 RNA copy number in pCTVØ (black circles) or pCTV-WS (red circles) immunized K18-hACE2 mice and challenged with the Wuhan strain at 5 and 7-12 DPI [n = 4–5, pCTVØ and n = 4–8 pCTV-WS]. Histopathological sections of lungs from pCTVØ e, g and pCTV-WS f, h immunized K18-hACE2 mice at 5 DPI and 7–12 DPI, respectively [n = 4 mice/group]. Inflammatory infiltrate (black arrow), perivascular inflammatory infiltrate (red arrow), alveolar septal thickening (yellow arrow), vascular congestion (blue arrow), edema (arrow head) and hemorrhage (green arrow) at 1.1×, 10×, and 20× magnification. The statistical analysis of weight curves was performed by area under the curve followed by unpaired t-test. The statistical analysis of survival was performed using the log-rank test. The statistical analysis of viral load was performed using unpaired t-test. *p < 0.05; ***p < 0.001.

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