Fig. 4: Inflammatory profile and immunohistochemical staining of B and T cells in lung samples from pCTV-WS immunized K18-hACE2 mice. | npj Vaccines

Fig. 4: Inflammatory profile and immunohistochemical staining of B and T cells in lung samples from pCTV-WS immunized K18-hACE2 mice.

From: Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concern

Fig. 4

Relative expression of cytokines a and chemokines b in lung samples of pCTVØ (black bar) or pCTV-WS (red bar) immunized mice and challenged with the Wuhan strain at 5 DPI [n = 4 mice/group]. Absolute number of B cells (CD19+), T cells (CD3+), CD4+ and CD8+ T cells c and inflammatory monocytes (F4/80+CD11b+Ly6Chigh), intermediate monocytes (F4/80+CD11b+Ly6Cint), monocytes (F4/80+CD11b+Ly6C-), monocyte-derived dendritic cells (F4/80+CD11b+Ly6ChighDC-Sign+), neutrophils (F4/80-CD11bhighLy6G+) and classical dendritic cells (CD11b-/lowCD11c+MHC-II+) d at 5 DPI in lung samples of pCTVØ (black bar) and pCTV-WS (red bar) immunized K18-hACE2 mice, by flow cytometry [n = 4 mice/group]. Immunohistochemical staining of lungs tissue sections from pCTVØ and pCTV-WS mice challenged with Wuhan strain stained with anti-CD4 e, f, anti-CD8 g, h, and anti-CD19 i, j showing the localization of CD4+ T cells, CD8+ T cells and B cells (red arrows) in lung tissue at 10×, 20×, and 40× magnification. The statistical analysis was performed using unpaired t-test. Bars represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.

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