Fig. 6: pCTV-WS immunogenicity and protective immunity in Syrian hamster model. | npj Vaccines

Fig. 6: pCTV-WS immunogenicity and protective immunity in Syrian hamster model.

From: Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concern

Fig. 6

a Schematic representation of the experimental design used to assess the humoral and cellular immune response and protection against SARS-CoV-2. b Anti-WS total IgG titer in pCTVØ (open circles) or pCTV-WS (gray circles) immunized hamsters after 1st immunization (prime) and pCTVØ (filled circle) or pCTV-WS (red circles) after prime/boost [n = 5, pCTVØ and n = 12, pCTV-WS]. c IgG1 (blue circles) and IgG2/IgG3 (red circles) subclass 30 days after prime/boost pCTV-WS immunization. IgG1 (open circle) and IgG2/IgG3 (filled circle) in the control group [n = 5, pCTVØ and n = 12, pCTV-WS]. d Anti-WS total IgG in bronchoalveolar lavage (BAL) of pCTVØ (black circles) or pCTV-WS (red circles) immunized hamsters [n = 10 mice/group]. e IFN-γ levels secreted by splenocytes from pCTVØ (black circles) or pCTV-WS (red circles) immunized hamsters after 72 h of incubation with medium (negative control), restimulation with recombinant WS protein or Concanavalin A (positive control). Lung viral load f and SARS-CoV-2 RNA copy number g in pCTVØ (black circles) or pCTV-WS (red circles) immunized hamsters and challenged with the Wuhan strain at 4 DPI [n = 5 mice/group]. Histopathological sections of lungs from pCTVØ h or pCTV-WS i immunized hamsters at 4 DPI. Inflammatory infiltrate (black arrow), perivascular inflammatory infiltrate (red arrow), alveolar septal thickening (yellow arrow), edema (arrow head) and hemorrhage (green arrow) at 1.1×, 10×, and 20× magnification. The statistical analysis was performed using unpaired t-test or Mann-Whitney U test, according to data distribution. **p < 0.01; ***p < 0.001.

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