Fig. 8: Neutralizing antibodies and protective immunity in pCTV-WS immunized K18-hACE2 mice. | npj Vaccines

Fig. 8: Neutralizing antibodies and protective immunity in pCTV-WS immunized K18-hACE2 mice.

From: Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concern

Fig. 8

Body weight a and survival b of K18-hACE2 mice after sera transfer from pCTVØ (black lines) or pCTV-WS (red lines) immunized mice followed by challenge with the Wuhan strain [n = 5, pCTVØ and n = 7 pCTV-WS]. Body weight c and survival d of pCTVØ (black lines) and pCTV-WS immunized K18-hACE2 mice, treated with αKLH (red lines) or αCD4/αCD8 (purple lines) and challenge with the Wuhan strain [n = 4 mice/group]. Statistical analysis: [*pCTVØ vs pCTV-WS (αKLH)] [#pCTVØ vs pCTV-WS (αCD4/αCD8)]. Body weight e and survival f of K18-hACE2 mice (black lines), receiving adoptive transfer of T cells (purple lines) or adoptive transfer of T cell + sera transfer (red lines) followed by challenge with Wuhan strain [n = 4 mice/group]. Statistical analysis: [*pCTVØ vs sera + T cell] [#sera + T cell vs T cell]. Body weight g and survival h of K18-hACE2 mice (black lines), and receiving sera transfer (green lines) followed by challenge with Delta variant [n = 4 mice/group]. Body weight i and survival j of pCTVØ (black lines) and pCTV-WS immunized K18-hACE2 mice, treated with αKLH (green lines) or αCD4/αCD8 (purple lines) and challenged with Delta variant [n = 3–4 mice/group]. Statistical analysis: [*pCTVØ vs pCTV-WS (αKLH)] [#pCTVØ vs pCTV-WS (αCD4/αCD8)]. Body weight k and survival l of K18-hACE2 mice (black lines), and receiving sera transfer (blue lines) followed by challenge with Gamma variant [n = 4 mice/group]. Body weight m and survival n of pCTVØ (black lines) and pCTV-WS immunized K18-hACE2 mice, treated with αKLH (blue lines) or αCD4/αCD8 (purple lines) and challenged with Gamma variant [n = 3–4 mice/group]. Statistical analysis: [*pCTVØ vs pCTV-WS (αKLH)] [#pCTVØ vs pCTV-WS (αCD4/αCD8)]. Body weight o and survival p of K18-hACE2 mice (black lines), receiving adoptive transfer of T cells (purple lines) or adoptive transfer of T cell + sera transfer (blue lines) followed by challenge with Gamma variant [n = 4 mice/group]. Statistical analysis: [*pCTVØ vs sera + T cell] [#pCTVØ vs T cell]. q SARS-CoV-2 RNA copy number from K18-hACE2 that received sera transfer from pCTVØ (black circles) or pCTV-WS (pink circles) immunized mice and challenged with the Omicron variant at 6 DPI [n = 4 mice/group]. r SARS-CoV-2 RNA copy number from K18-hACE2 mice immunized with pCTVØ (black circles) and pCTV-WS that received isotype control antibody (αKLH - pink circles) or αCD4/αCD8 (purple circles) and challenged with the Omicron variant at 6 DPI [n = 4 mice/group]. s Viral RNA load of K18-hACE2 mice that received adoptive T cell transfer (purple circles) or adoptive T cell transfer + sera transfer (pink circles) and challenged with the Omicron variant at 6 DPI [n = 4 mice/group. PRNT50 of K18-hACE2 mice immunized with pCTVØ (black circles) or pCTV-WS (colored circles) before and after challenge with Wuhan strain t or with Gamma and Omicron variants u [n = 4–6 mice/group]. Body weight v and survival w of K18-hACE2/B-KO mice immunized with pCTVØ (black lines) or pCTV-WS (red lines) and challenged with the Wuhan strain [n = 3–9 mice/group]. x Viral RNA load of K18-hACE2/B-KO mice immunized with pCTVØ (black circles) or pCTV-WS (pink circles) and challenged with Omicron variant [n = 3 mice/group]. Data pooled from one or two independent experiments. The statistical analysis of weight curves was performed by area under curve followed by unpaired t-test or Mann-Whitney U test, according to the data distribution. The statistical analysis of survival was performed using the log-rank test. The statistical analysis for RNA copy number was performed using unpaired t-test or Mann-Whitney U test, according to data distribution. *p < 0.05; **p < 0.01; ***p < 0.001.

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