Fig. 7: Evaluation of protection against T. cruzi infection. | npj Vaccines

Fig. 7: Evaluation of protection against T. cruzi infection.

From: ASP-2/Trans-sialidase chimeric protein induces robust protective immunity in experimental models of Chagas’ disease

Fig. 7

The parasite load in the heart was measured by qPCR (a) and the apex section, the most parasite-abundant, was selected to investigate the difference between the groups (b, c). It was analyzed the percentage of positive animals (b) and the number of parasites/100 ng of gDNA (c). Histopathological analysis showed an increase in inflammatory process in non-vaccinated dogs, represented by the number of cells per microscope field (d) and photomicrographs of the groups PBS (e), Poly-ICLC (f), DNA∅ + AdβGal (g), TRASP-pICLC (h), and DNA TS/ASP + Ad TS/ASP (i). a, d Mean ± SEM, c median ± range. The statistical analysis of the parasite load was assessed by Mann–Whitney test and histopathology was analyzed by one-way ANOVA and Tukey’s multiple test. n = 6–9 dogs per group. *p < 0.05; **p < 0.01.

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