Fig. 1: Cattle immunization. | npj Vaccines

Fig. 1: Cattle immunization.

From: Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates parasite transmission by Rhipicephalus microplus ticks

Fig. 1

SDS–PAGE (A) and immunoblot (B) analysis of Ni-column purified B. bovis rHAP2 using mouse anti-6His antibody. The rHAP-2 protein is identified by arrows. The size of protein markers is indicated on the left. C Serological analysis by iELISA for the detection of anti-rHAP2 antibodies on rHAP2 vaccinated calves (1646, 1647, and 1649), and non-vaccinated controls (1650, 1652, and 1653). The Y and X axes represent absorbance at 450 nm and days after vaccination (days 0–94), respectively. The red dashed line represents the cutoff value of the iELISA, calculated as the OD of non-infected cattle sera +3 standard deviations. The error bars represent the standard deviations of samples at each time point.

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