Fig. 5: Assessment of B. bovis transmission by R. microplus larvae derived from vaccinated and non-vaccinated cattle. | npj Vaccines

Fig. 5: Assessment of B. bovis transmission by R. microplus larvae derived from vaccinated and non-vaccinated cattle.

From: Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates parasite transmission by Rhipicephalus microplus ticks

Fig. 5

A Detection of B. bovis DNA by quantitative PCR (qPCR) based on the B. bovis msa-1 gene on DNA extracted from blood from calves receiving ticks derived from vaccinated (C99555; C99556, C99557), or non-vaccinated control (C99559, C99561, C99562) calves. The Y-axis represents the gene number copies detected. The X-axis represents the timing for the extraction of each blood sample (days post-tick infestation of the cattle). B Detection of antibodies against the B. bovis RAP-1 protein by iELISA on sera from calves receiving ticks derived from vaccinated (C99555; C99556, C99557), or non-vaccinated control (C99559, C99561, C99562) calves. C37816 represents iELISA analysis performed sera from a calf experimentally infected with B. bovis. BABB75 represents iELISA performed with a monoclonal antibody reactive with RAP-1. The Y-axis represents OD at 435 nm. The red dashed line represents the cutoff value of the iELISA, calculated as the OD of non-infected cattle sera +3SD). The error bars in Panel A represent standard deviations of the number of msa-1 copies in experimental animals. The error bars in Panel B represent standard deviations of the OD 450 nm values in experimental animals.

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