Fig. 1: Construction and characterization of AdC68-G and DNA-G. | npj Vaccines

Fig. 1: Construction and characterization of AdC68-G and DNA-G.

From: Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection

Fig. 1

a Schematic diagram of the genome of AdC68-G and the coding sequence for the NiV G protein. b Diagram of plasmid DNA-G. The plasmid pVAX1 vector was used to engineer a gene cassette containing a CMV promoter, the Kozak sequence, NiV-G cDNA, and a BGH pA signal element. c The cytopathic effect (CPE) was observed in HEK293 cells transfected with the linearized AdC68-G or AdC68 plasmid. d Western blot analysis of HEK293 cells infected with rescued recombinant AdC68-G or AdC68 viruses. e Western blot analysis of HEK293T cells transfected with DNA-G (pVAX1-G) or empty vector (pVAX1). GAPDH was used as the internal control. All blots derive from the same experiment and that they were processed in parallel. Bar scale, 100 µm.

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