Fig. 3: Immunogenicity and challenge schedule in hamsters. | npj Vaccines

Fig. 3: Immunogenicity and challenge schedule in hamsters.

From: Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection

Fig. 3

a The experimental scheme of Syrian hamsters. The animals were vaccinated at day 0 with AdC68-G (via the i.n. or i.m. route) or DNA-G (i.m./EP) and boosted with AdC68-G (via the i.n. or i.m. route) after 21 days (the upper) or immunized 3 times with DNA-G via the i.m./EP route (the lower) and then challenged with the NiV Malaysia and Bangladesh strains at 1000 LD50 via the i.p. route. Hamsters were monitored daily for survival as well as body weight changes. Six hamsters in each group (except for those in the AdC68-G (i.n.) group that was challenged with NiV-M, n = 3) were euthanized at 5 d.p.i. for viral load and pathogenesis analysis. The IgG antibodies (b) and pNAbs (c) on day 21 after the first or second vaccination. The IgG antibodies (d) and pNAbs (e) on day 21 after the first, second and third vaccinations. f vNAb detection based on the live NiV Malaysia and Bangladesh strains at 3 weeks after the last immunization. Data are presented as the group mean ± SEM. Statistical significance was determined one-way ANOVA with Tukey’s multiple comparisons tests. n.s., not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate 95% confidence intervals. L.O.D. represents the limit of detection.

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