Fig. 5: T cell response measured after 12 days of primary immunization. | npj Vaccines

Fig. 5: T cell response measured after 12 days of primary immunization.

From: Intranasal immunization of mice with chimera of Salmonella Typhi protein elicits protective intestinal immunity

Fig. 5

a Th1 (IL-12, IFN-γ), Th2 (IL-4, IL-5), and Treg (IL-10) cell cytokines in the serum samples of the unimmunized and immunized mice were measured by ELISA. b Cells from the PP of the experimental mice were incubated with 10 µg of T2544 for 24 h. Cytokine-secreting cells were enumerated by ELISpot assays and data presented as spot forming cells/106 total cells analyzed. c Analysis of TFH (T follicular helper) cells in the MLN. Cells were detected by measuring the expression of CXCR5 and PD-1 on CD4+ cells using flow cytometer. Significance was calculated by one way (b, c) and two-way (a) ANOVA with post hoc analysis using Tukey’s multiple comparison test. The experiment was repeated three times and representative images from one experiment are shown. Bar diagram represents the statistical data from all three experiments. Error bars represent SD. ****p < 0.0001, ***p = 0.0006.

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