Fig. 1: Design and construction of the combination vaccine AdC68-HATRBD. | npj Vaccines

Fig. 1: Design and construction of the combination vaccine AdC68-HATRBD.

From: An intranasal combination vaccine induces systemic and mucosal immunity against COVID-19 and influenza

Fig. 1

a Diagram of AdC68-HATRBD construction. odRBD, Omicron-Delta chimeric RBD dimer; baRBD, Beta-Alpha chimeric RBD dimer; TCEs, tandem conserved T-cell epitopes from ORF1,ORF3, and M proteins of SARS-CoV-2. b The sequence of baRBD or odRBD was subjected to AlphaFold2 server for tertiary structure prediction, and analyzed using PyMol. In baRBD dimer, Beta RBD subunit is shown in orange with Alpha RBD in green. In odRBD dimer, Omicron RBD subunit is shown in grey with Delta RBD in pink. c Western blot analysis of expression of RBD dimers and HA in AdC68-HATRBD transduced HEK 293 cell lysates under reductive conditions. AdC68-empty was included as negative control.

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