Fig. 1: Western blot analysis of NDV-PFS. | npj Vaccines

Fig. 1: Western blot analysis of NDV-PFS.

From: Intranasal vaccination with an NDV-vectored SARS-CoV-2 vaccine protects against Delta and Omicron challenges

Fig. 1

Western blot analysis of spike protein expression from uninfected (A), NDV-GFP (B) or NDV-PFS (C) infected cell lysates (100 μg of protein) and 5 × 104 PFU of NDV-PFS (D) or NDV-GFP (E) purified viral stocks. Full-length spike protein (180 kDa) was detected using an anti-S1 antibody, with infection confirmed by the presence of NDV ribonucleoprotein (RNP) complex. DF1 cells were infected at an MOI of 1 for 24 h with either NDV-GFP, NDV-PFS, or left uninfected to generate cell lysates. Full-length spike protein expression is evident following NDV-PFS infection and on the surface of NDV-PFS compared to NDV-GFP, NDV-GFP-infected cells, and uninfected cells. The blots shown were processed in parallel for the detection of either SARS-CoV-2 or NDV.

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