Fig. 6: Cell-mediated immune response to NDV-PFS in K18 mice. | npj Vaccines

Fig. 6: Cell-mediated immune response to NDV-PFS in K18 mice.

From: Intranasal vaccination with an NDV-vectored SARS-CoV-2 vaccine protects against Delta and Omicron challenges

Fig. 6

Equal numbers of male and female K18 mice were not vaccinated (n = 6) or vaccinated with one (n = 6) or two (n = 6) doses of NDV-PFS and spleens and lungs were harvested 10 days or 5 days post-administration, respectively. Isolated cells were stimulated with ancestral spike peptides for 18 h prior to staining. Following the identification of live activated T cells (CD25+, OX40+), populations were further separated into CD4 (left panels) and CD8 T cells (right panels) (CD25+, OX40+, CD3+ CD4/CD8+) and assessed for polyfunctionality (IFNγ+ and TNFα+) in the lung (top panels) and spleen (bottom panels). Shown are mean values + SD. Significance was assessed by one-way analysis of variance and Tukey’s multiple comparisons test. Exact p values are shown where significant differences were seen.

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