Fig. 3: S-specific B-cells in hybrid kidney disease patients and controls display subtle phenotypical variations. | npj Vaccines

Fig. 3: S-specific B-cells in hybrid kidney disease patients and controls display subtle phenotypical variations.

From: SARS-CoV-2-specific immune responses converge in kidney disease patients and controls with hybrid immunity

Fig. 3

a t-SNE-based dimensional reduction plots of concatenated B-cells in region 1 (Fig. 2a). Clusters identified by unsupervised cluster analysis using FlowSOM are numbered in the center of each cluster and color coded according to their phenotypic resemblance; CD27+ B-cells with low Ig expression (cluster 9,10; greys), class-switched CD27+ memory populations (cluster 6-8; yellow/greens), CD62L+ lymph node homing (cluster 3–5; blues) and CD27– extrafolicular memory populations (cluster 1,2; orange and red). b S-specific B-cells of hybrid controls, CKD and dialysis patients, and KTR overlayed on the t-SNE plot, color coded per cluster. c) Distribution of isotype, memory and lymph node homing marker expression shown as the mean fluorescent intensity (MFI) on the t-SNE map. d Frequency of S-specific B-cells within region 1. The central line, box, and whiskers represent the mean, interquartile range, and minimum or maximum values, respectively. e Relative mean frequencies (boxes) and standard error of mean (whiskers) of S-specific B-cell phenotypes in region 1, color-coded similar to panel a. Samples without S-specific B-cells in region 1 were omitted from this analysis.

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