Fig. 2: Dose optimization of gH/gL LION/repRNA. | npj Vaccines

Fig. 2: Dose optimization of gH/gL LION/repRNA.

From: A gH/gL-encoding replicon vaccine elicits neutralizing antibodies that protect humanized mice against EBV challenge

Fig. 2

a Kinetics of reciprocal gH/gL endpoint binding titers. Half-maximal neutralizing titers against EBV infection of epithelial (b) or B cells (c) following equal doses of LION/repRNA encoded gH/gL delivered at weeks 0 and 8 as indicated. The gH/gL binding (d) and EBV neutralizing titers measured in epithelial (e) and B cell (f) infection assays following two unequal doses of repRNA encoded gH/gL delivered at weeks 0 and 8 as indicated. Each data point represents the average of two technical replicates for an individual mouse at each timepoint (n = 4 per group for equal 0.1 and 1 µg prime/boost doses, n = 8 for equal 10 µg prime/boost doses, and n = 5 per group for mixed dose prime/boost) and lines connect the means. Arrows indicate the time of immunization. See Table S1 for statistical comparisons.

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