Fig. 3: The construction of a novel TMUV DNA vaccine expressing prM-E together with dusCD40L.
From: Duck CD40L as an adjuvant enhances systemic immune responses of avian flavivirus DNA vaccine
a Schematic diagram of the TMUV prM-E DNA vaccine containing dusCD40L. The DEFs were transfected with the prM-E-based three plasmids, respectively. The expression of E and dusCD40L were measured by IFA (b) and WB (c) assays at 48 h post the plasmids transfection. Scale bars, 40 μm. d The expression of E protein was measured in vivo. Ducks were immunized with 200 μg of pVAX1, pVAX-prM-E, or pVAX-prM-E-dusCD40L by intramuscular injection. They were sacrificed at 7 days post-immunization. The spleens were collected and the E protein was detected by immunohistochemistry assay. The black arrows indicate the E proteins. Scale bars, 20 μm.
