Fig. 5: TMUV-specific T cell responses post-vaccination.
From: Duck CD40L as an adjuvant enhances systemic immune responses of avian flavivirus DNA vaccine
a Schematic diagram of the vaccination and sampling procedure. b The proliferation of duPBMCs was detected by CCK-8 assay. DuPBMCs were seeded into a 96-well plate, 5×105 cells/well, and stimulated with 100 μg/mL inactivated TMUV. The relative proliferation rate = vaccine (OD450 TMUV stimulation - OD450 mock) / OD450 mock × 100%. c The IFNγ titers were detected by ELISA. Data were presented as means ± s.d. (n = 3). Statistical significance was assessed using an unpaired Student’s t-test. ns > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.
