Fig. 5: Design, validation, production and characterisation of a hyper stable multi-presenting antigen. | npj Vaccines

Fig. 5: Design, validation, production and characterisation of a hyper stable multi-presenting antigen.

From: A rationally designed antigen elicits protective antibodies against multiple nosocomial Gram-positive pathogens

Fig. 5

A Molecular model of Sc(EH)3 after energy minimisation. B DiscoTope predictions of Sc(EH)3, showing predicted antigenicity solely for the inserted EH-motifs. C Root-mean-square deviations (RMSD) computed on Cα atoms, reported for either the core or the EH-motifs residues for the three MD simulations at three increasing velocities in black, red, and green. D Root-mean-square fluctuations (RMSF) on Cα atoms showing high flexibility solely for the three EH-motifs. E Far-UV CD spectra were measured at 0.2 mg mL−1 in 20 mM sodium phosphate buffer (pH 7.4) after incubation for 1 h at 20 °C (green), 37 °C (blue) and 100 °C (black). F Thermal denaturation curves of Sc(EH)3 monitored at 222 nm in 20 mM sodium phosphate buffer (black) and in the presence of 3 M GuHCl denaturant (red). G Resistance to storage at 37 °C for 30 days of Sc(EH)3 compared to AdcA, as shown by SDS-gel electrophoresis. Degradation bands of AdcA are highlighted by arrows.

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