Fig. 6: OPKA and OPIA assays against E. faecium 11236/1, S.aureus MW2 and E. faecalis T2.
OPKA assays against (A) E. faecium 11236/1, (B) S. aureus MW2, and (C) E. faecalis T2. OPKA killing percentages mediated by anti-Sc(EH)3 sera are compared to those mediated by anti-AdcA (black bars). The statistical significance was tested by the unpaired two-tailed T test with a 95% confidence interval with a Bonferroni post hoc test using anti-AdcA and anti-Sc(EH)3 sera at the same concentration. Bars and whiskers denote mean values ± standard errors of the mean. *P ≤ 0.0125, **P ≤ 0.01, ***P ≤ 0.001. OPIA assays with 0.4 mg/mL anti-AdcA or anti-Sc(EH)3 sera against E. faecium 11236/1 (D–G) S. aureus MW2 (H–K) and E. faecalis T2 (L–O) by addition of different inhibitors. Anti-AdcA and anti-Sc(EH)3 were pre-incubated with different concentrations of inhibitors, including recombinant AdcA (light blue bars), ZnuA domain (light grey bars), and recombinant Sc(EH)3 (dark grey bars). Anti-AdcA sera incubated with AdcA were used as positive controls (D, H, L). As negative controls (0) for each plot, we used sera at 0.4 mg/mL anti-AdcA (dark blue bar) and anti-Sc(EH)3 (black bars) without inhibitors. Statistical significance of inhibition was performed by the One way analysis of variances test, followed by the Dunnett’s multiple comparison post hoc test. Bars and whiskers denote mean values ± standard errors of the mean of replicates within one assay. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
