Fig. 8: In vivo active protective efficacy of Sc(EH)3 and AdcA antigens against E. faecium, E. faecalis and S. aureus.
A IgG responses of mice immunised with Sc(EH)3 antigen in each group (S. aureus MW2, E. faecium 11236/1 and E. faecalis T2) (n = 10 per group), after two subcutaneous doses (after 2 weeks) and two intraperitoneal doses (after 4 weeks), compared to the control group receiving only buffer and IFA (n = 10). B As in panel (A), IgG responses of mice immunised with AdcA antigen+IFA (n = 10 per group) compared to the control group (n = 10). C Percentage of survived mice during the active protection assay against 9 × 109 CFU of E. faecium 11236/1, (D) against 7 × 109 CFU of S. aureus MW2 and (E) against 6.5 × 109 CFU of E. faecalis T2. The discontinuous blue line represents the group treated with Sc(EH)3 antigen (n = 10), the green line the group treated with AdcA antigen (n = 10) and the black line the untreated control group (n = 10). Statistical differences between groups were assessed using Log Rank (Mantel-cox test). Difference between the treated groups with Sc(EH)3 antigen and the control groups are marked by star symbol, ns: not significant; *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Difference between the treated groups with AdcA antigen and the control groups are marked by hash symbol, ns: not significant; # : p < 0.05, # # : p < 0.01, # # # : p < 0.001 and # # # # : p < 0.0001.
