Fig. 3: CircRNA vaccination induced NA-specific cellular responses. | npj Vaccines

Fig. 3: CircRNA vaccination induced NA-specific cellular responses.

From: CircRNA based multivalent neuraminidase vaccine induces broad protection against influenza viruses in mice

Fig. 3

a–c Mice were vaccinated with 10 μg of circRNA-LNP encoding N1, N2, or IBV NA, following a prime-boost vaccination scheme. Ten days post boost vaccination, the spleen was harvested and spot-counting for IFN-γ/IL-4 -secreting cells by ELISpot assay after stimulating with N1 (a), N2 (b) or IBV NA (c) recombinant protein. Representative walls of IFN-γ (d) or IL-4 (e) ELISpot. The proportion of IFN-γ (f), TNF-α (g), or IL-2 (h) secreting CD4+ and CD8+ T cells was assessed by FASC. Splenocytes from the blank LNP group were used as a control. Data are presented as the mean ± SD (n = 5), and each symbol represents one animal. Statistical significance was performed by unpaired t test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant.

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