Fig. 5: PfRh5-associated genetic diversity in P. falciparum clinical isolates from Kédougou (N = 22). | npj Vaccines

Fig. 5: PfRh5-associated genetic diversity in P. falciparum clinical isolates from Kédougou (N = 22).

From: Vaccine-induced human monoclonal antibodies to PfRH5 show broadly neutralizing activity against P. falciparum clinical isolates

Fig. 5

A Summary table showing the multiplicity of infections (MOI) per isolate determined through msp1-2 typing as well as the number of SNPs at each position (bold characters) and their respective read frequencies (shown in brackets). B The prevalence of PfRH5-associated SNPs was calculated as the percentage of SNPs detected within the total phenotyped sample population (N = 22). PfRH5 sequencing was performed from pfrh5 amplicons using the Illumina NovaSeq 6000 sequencing platform and variant analysis was performed using the Geneious Prime software version 23.1.1. SNPs displayed to the right of the dotted line represent SNPs present in a single sample. C SNP variant read frequencies in genomically complex samples were determined from the sequencing data outputs and calculated as the percentage of the variant read coverage relative to the coverage at the variant position. The red dotted line depicts the 2% threshold for variant allele frequency. The graphs were plotted using the GraphPad Prism version 1.0.2 software.

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