Fig. 2: CD8⁺ T-cell subpopulations in the lung.

Balb/c mice were immunized as described before and lymphocytes from lungs were examined 36 days post boost. Antigen experienced CD8⁺ T-cells were identified by F_85-93-specific pentamer staining and intravascular (iv) CD45 pan staining. CD45-labelled cells (iv⁺) were defined as circulating and iv protected (iv^-) as tissue resident memory cells. a The absolute number of F_85-93⁺ CD8⁺ with the relative contribution of iv⁺ and iv^- cells was summarized for each group. Statistical analysis was performed over whole population. b Within the iv⁺ F_85-93⁺ CD8⁺ population central memory T-cells (T_CM; CD127⁺KLRG1^-CD69^-CD103^-), effector T-cells (T_EFF; CD127^-KLRG1⁺) and effector memory T-cells (T_EM; CD127⁺KLRG1⁺) were determined, whereas within the iv^- population tissue resident memory T-cells (T_RM; KLRG1^-CD103⁺CD69⁺ or KLRG1^-CD103⁺CD69^- or KLRG1^-CD103^-CD69⁺) were defined¹⁰⁰. Bars represent mean values with +SEM; naive n = 3, rAd5 (i.n.) n = 5, other groups n = 6. Data were analysed by one-way ANOVA followed by Tukey´s multiple comparison test. Statistically significant differences were indicated only among the used vaccine vectors within the intranasal resp. intramuscular group (*p < 0.05; **p < 0.005).