Fig. 3: F-specific T-cell responses after immunization in lung and spleen. | npj Vaccines

Fig. 3: F-specific T-cell responses after immunization in lung and spleen.

From: Evaluation of adenoviral vector Ad19a encoding RSV-F as novel vaccine against respiratory syncytial virus

Fig. 3

Balb/c mice were immunized as described before and lymphocytes from lungs and spleens were restimulated with peptide pools covering immunogenic parts of F 36 days post boost. CD8+ (a and b) and CD4+ (c and d) T-cell responses were analysed by staining for the degranulation marker CD107a and intracellular staining for inflammatory cytokines IFNγ, TNFα and IL2. a, c Lung responses of tissue resident (iv-) T-cells were analysed. Total numbers of different populations among CD8+ and CD4+ T-cells are shown. Bars represent mean values with +SEM; naive n = 3, rAd5 (i.n.) n = 5, other groups n = 6. Data were analysed by one-way ANOVA followed by Tukey´s multiple comparison test. Statistically significant differences were indicated only among the used vaccine vectors within the intranasal resp. intramuscular group (*p < 0.05; **p < 0.005). poly: polyfunctional T-cell populations positive for all assessed markers.

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