Table 1 Comparison of reverse genetics system rescue frequencies for different RV strains using Kanai et al., Sánchez-Tacuba et al., and the current improved system

From: CRISPR/Cas9 screens identify key host factors that enhance rotavirus reverse genetics efficacy and vaccine production

Recombinant RV strains

Rescue efficiency

Kanai et al.3

Sánchez-Tacuba et al.4

Current system (C3P3-G1)

Current system (C3P3-G3)

rRRV

0/3

5/6

N.T.

6/7

rD6/2-2 g

0/3

4/5

N.T.

6/8

rSA11-RotaTeq-VP4

0/3

0/3

1/6

1/3

rD6/2-2g-NSP6-Del-NLuc

0/3

0/3

4/6

3/3

  1. The first number in parenthesis represents the number of successful rescues, and the second number in parenthesis represents the total number of attempts.
  2. N.T. not tested.
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