Fig. 5: S and N DNA vaccines elicit long-term IgG and nAb responses in a SARS-CoV-2 variant-specific manner in K18-hACE2 mice. | npj Vaccines

Fig. 5: S and N DNA vaccines elicit long-term IgG and nAb responses in a SARS-CoV-2 variant-specific manner in K18-hACE2 mice.

From: Co-immunization with spike and nucleocapsid based DNA vaccines for long-term protective immunity against SARS-CoV-2 Omicron

Fig. 5

a Experimental protocol: On days 0 and 21, K18-hACE2 mice were inoculated with S and/or N DNA vaccines or pVAX control (25 µg, IM) and blood was harvested on days 60, 115, and 140. Representation of the experiment timeline was Created with BioRender.com. b–d ELISA assays showing endpoint titers of IgG against N, S, or RBD proteins from the indicated SARS-CoV-2 strains. Data are from 2 independent experiments (n = 9–10 mice/group). e Neutralization assay (pVNT50) using pseudoviruses harboring S proteins from the indicated SARS-CoV-2 strains. Data are from 2 independent experiments (n = 9-11 mice/group). b-e Serum samples were tested in duplicate on the same plates. Group means were compared by the non-parametric Kruskal-Wallis test. *P < 0.05, **P < 0.01, ***P < 0.001. Circles, individual mice; dashed lines, detection limit.

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