Fig. 1: repNP + repGc vaccination elicits humoral and cellular immunity up to 1 year post-vaccination. | npj Vaccines

Fig. 1: repNP + repGc vaccination elicits humoral and cellular immunity up to 1 year post-vaccination.

From: Replicating RNA vaccine confers durable immunity against Crimean Congo hemorrhagic fever virus challenge in mice

Fig. 1

WT C57BL6/J mice were vaccinated prime boost with 1 µg repNP + repGc RNA 4 weeks apart. Immunogenicity of vaccination was measured 1-, 3-, 6-, 9-, and 12-months post-boost vaccination. At each time point, groups of six mice were evaluated for CCHFV specific immune responses via a whole virion IgG ELISA shown with endpoint titers and b recombinant antigen ELISA to the CCHFV nucleocapsid (NP) and glycoprotein c (Gc) proteins. Sham mice are combined from all timepoints. Dashed lines indicate background absorbance of wells. CCHFV-specific T-cell responses were measured via IFNγ ELISpot shown as c cumulative spot-forming cells (SFCs) against peptide pools spanning the entire NP or Gc as well as d cumulative responses to NP and Gc overtime. Statistical comparisons were calculated using a one-way ANOVA with Turkey’s multiple comparison test. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are shown as mean plus standard deviation.

Back to article page