Fig. 4: Anamnestic responses to CCHFV infection. | npj Vaccines

Fig. 4: Anamnestic responses to CCHFV infection.

From: Replicating RNA vaccine confers durable immunity against Crimean Congo hemorrhagic fever virus challenge in mice

Fig. 4

Surviving mice from repNP + repGc 6-, 9-, and 12-months groups were evaluated on day 14 p.i. for CCHFV-specific anamnestic responses. Sera was collected and evaluated for CCHFV-specific antibodies using a recombinant antigen ELISA to the CCHFV NP, Gn, GP38, and Gc. Responses are shown for the 6-, 9-, and 12-month groups as well as comparisons between D0 and D14 for CCHFV-NP and Gc responses. Further, anamnestic CCHFV-specific T-cell responses were measured via b IFNγ ELISpot shown as cumulative SFCs against peptide pools spanning the entire GPC or NP as well as a comparison between D0 and D15 responses to the GPC peptide pool 10. Data from day 0 is duplicated from Fig. 1 and shown here for comparison. Dashed lines indicate limit of detection. Statistical comparisons were calculated using a one-way ANOVA with Turkey’s multiple comparison test. ns >0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are shown as mean plus standard deviation.

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